|
View: |
Part 1: Document Description
|
|
Citation |
|
|---|---|
|
Title: |
Perampanel ameliorates nitroglycerin-induced migraine through inhibition of the cAMP/ PKA/CREB signaling pathway in the trigeminal ganglion in rats |
|
Identification Number: |
doi:10.7910/DVN/Y7VYUQ |
|
Distributor: |
Harvard Dataverse |
|
Date of Distribution: |
2023-06-27 |
|
Version: |
1 |
|
Bibliographic Citation: |
QingLing Zhai; KaiXin Wang; Defu Zhang; Jinbo Chen; XiaoMeng Dong; Yonghui Pan, 2023, "Perampanel ameliorates nitroglycerin-induced migraine through inhibition of the cAMP/ PKA/CREB signaling pathway in the trigeminal ganglion in rats", https://doi.org/10.7910/DVN/Y7VYUQ, Harvard Dataverse, V1 |
|
Citation |
|
|
Title: |
Perampanel ameliorates nitroglycerin-induced migraine through inhibition of the cAMP/ PKA/CREB signaling pathway in the trigeminal ganglion in rats |
|
Identification Number: |
doi:10.7910/DVN/Y7VYUQ |
|
Authoring Entity: |
QingLing Zhai (Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, China) |
|
KaiXin Wang (Department of Neurology, The Third Hospital of Jinan Shandong, China) |
|
|
Defu Zhang (Department of Neurology, Shengli Oilfield Central Hospital, Shandong, China) |
|
|
Jinbo Chen (Department of Neurology, Binzhou Medical University Hospital, Shandong, China) |
|
|
XiaoMeng Dong (Department of Neurology, Binzhou Medical University Hospital, Shandong, China) |
|
|
Yonghui Pan (Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, China) |
|
|
Distributor: |
Harvard Dataverse |
|
Access Authority: |
Yonghui Pan |
|
Depositor: |
KJP, Office |
|
Date of Deposit: |
2023-06-27 |
|
Holdings Information: |
https://doi.org/10.7910/DVN/Y7VYUQ |
|
Study Scope |
|
|
Keywords: |
Medicine, Health and Life Sciences, Chronic Pain, Cyclic AMP-Dependent Protein Kinases, Glutamate, Hippocampus, Hyperalgesia, Migraine Disorders, Nitroglycerin, Perampanel, Pituitary Adenylate Cyclase-Activating Polypeptide, Rats, Receptors, AMPA |
|
Abstract: |
Perampanel, a highly selective glutamate AMPA receptor antagonist, is widely used to treat epilepsy. Since the existence of common pathophysiological features between epilepsy and migraine, the aim of this study was to investigate whether perampanel could exert an antimigraine effect. Nitroglycerin (NTG) was used to induce a migraine model in rats, and the model animals were pretreatment with 50 μg/kg and 100 μg/kg perampanel. The expression of pituitary adenylate-cyclase-activating polypeptide (PACAP) was quantified by western blot and quantitative real-time PCR in the trigeminal ganglion, and rat-specific enzyme-linked immunosorbent assay in serum. Western blot was also conducted to explore the effects of perampanel treatment on the phospholipase C (PLC)/protein kinase C (PKC) and protein kinase A (PKA)/ cAMP-responsive-element-binding protein (CREB) signaling pathways. Moreover, the cAMP/PKA/CREB-dependent mechanism was evaluated via in vitro stimulation of hippocampal neurons. The cells were treated with perampanel, antagonists and agonists for 24 hours and cell lysates were prepared for western blot analysis. |
|
Methodology and Processing |
|
|
Sources Statement |
|
|
Data Access |
|
|
Other Study Description Materials |
|
|
Related Publications |
|
|
Citation |
|
|
Identification Number: |
https://doi.org/10.3344/kjp.23039 |
|
Bibliographic Citation: |
Korean J Pain 2023;36(3):346-357 |
|
Label: |
KJP-23-039.xlsx |
|
Notes: |
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet |