{"@context":{"@language":"en","@vocab":"https://schema.org/","citeAs":"cr:citeAs","column":"cr:column","conformsTo":"dct:conformsTo","cr":"http://mlcommons.org/croissant/","rai":"http://mlcommons.org/croissant/RAI/","data":{"@id":"cr:data","@type":"@json"},"dataType":{"@id":"cr:dataType","@type":"@vocab"},"dct":"http://purl.org/dc/terms/","examples":{"@id":"cr:examples","@type":"@json"},"extract":"cr:extract","field":"cr:field","fileProperty":"cr:fileProperty","fileObject":"cr:fileObject","fileSet":"cr:fileSet","format":"cr:format","includes":"cr:includes","isLiveDataset":"cr:isLiveDataset","jsonPath":"cr:jsonPath","key":"cr:key","md5":"cr:md5","parentField":"cr:parentField","path":"cr:path","recordSet":"cr:recordSet","references":"cr:references","regex":"cr:regex","repeated":"cr:repeated","replace":"cr:replace","sc":"https://schema.org/","separator":"cr:separator","source":"cr:source","subField":"cr:subField","transform":"cr:transform","wd":"https://www.wikidata.org/wiki/"},"@type":"sc:Dataset","conformsTo":"http://mlcommons.org/croissant/1.0","name":"Perampanel ameliorates nitroglycerin-induced migraine through inhibition of the cAMP/ PKA/CREB signaling pathway in the trigeminal ganglion in rats","url":"https://doi.org/10.7910/DVN/Y7VYUQ","creator":[{"@type":"Person","affiliation":{"@type":"Organization","name":"Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, China"},"name":"QingLing Zhai"},{"@type":"Person","affiliation":{"@type":"Organization","name":"Department of Neurology, The Third Hospital of Jinan Shandong, China"},"name":"KaiXin Wang"},{"@type":"Person","affiliation":{"@type":"Organization","name":"Department of Neurology, Shengli Oilfield Central Hospital, Shandong, China"},"name":"Defu Zhang"},{"@type":"Person","affiliation":{"@type":"Organization","name":"Department of Neurology, Binzhou Medical University Hospital, Shandong, China"},"name":"Jinbo Chen"},{"@type":"Person","affiliation":{"@type":"Organization","name":"Department of Neurology, Binzhou Medical University Hospital, Shandong, China"},"name":"XiaoMeng Dong"},{"@type":"Organization","parentOrganization":{"@type":"Organization","name":"Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, China"},"name":"Yonghui Pan"}],"description":"Perampanel, a highly selective glutamate AMPA receptor antagonist, is widely used to treat epilepsy. Since the existence of common pathophysiological features between epilepsy and migraine, the aim of this study was to investigate whether perampanel could exert an antimigraine effect. Nitroglycerin (NTG) was used to induce a migraine model in rats, and the model animals were pretreatment with 50 μg/kg and 100 μg/kg perampanel. The expression of pituitary adenylate-cyclase-activating polypeptide (PACAP) was quantified by western blot and quantitative real-time PCR in the trigeminal ganglion, and rat-specific enzyme-linked immunosorbent assay in serum. Western blot was also conducted to explore the effects of perampanel treatment on the phospholipase C (PLC)/protein kinase C (PKC) and protein kinase A (PKA)/ cAMP-responsive-element-binding protein (CREB) signaling pathways. Moreover, the cAMP/PKA/CREB-dependent mechanism was evaluated via in vitro stimulation of hippocampal neurons. The cells were treated with perampanel, antagonists and agonists for 24 hours and cell lysates were prepared for western blot analysis.","keywords":["Medicine, Health and Life Sciences","Chronic Pain","Cyclic AMP-Dependent Protein Kinases","Glutamate","Hippocampus","Hyperalgesia","Migraine Disorders","Nitroglycerin","Perampanel","Pituitary Adenylate Cyclase-Activating Polypeptide","Rats","Receptors, AMPA"],"license":"http://creativecommons.org/publicdomain/zero/1.0","datePublished":"2023-06-27","dateModified":"2023-06-27","includedInDataCatalog":{"@type":"DataCatalog","name":"Harvard Dataverse","url":"https://dataverse.harvard.edu"},"publisher":{"@type":"Organization","name":"Harvard Dataverse"},"version":"1.0","citeAs":"@data{DVN/Y7VYUQ_2023,author = {QingLing Zhai and KaiXin Wang and Defu Zhang and Jinbo Chen and XiaoMeng Dong and Yonghui Pan},publisher = {Harvard Dataverse},title = {Perampanel ameliorates nitroglycerin-induced migraine through inhibition of the cAMP/ PKA/CREB signaling pathway in the trigeminal ganglion in rats},year = {2023},url = {https://doi.org/10.7910/DVN/Y7VYUQ}}","citation":[{"@type":"CreativeWork","name":"Korean J Pain 2023;36(3):346-357"}],"distribution":[{"@type":"cr:FileObject","@id":"KJP-23-039.xlsx","name":"KJP-23-039.xlsx","encodingFormat":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","md5":"061538c0e62226ac2f3ff787bfe19bf5","contentSize":"39241","description":"","contentUrl":"https://dataverse.harvard.edu/api/access/datafile/7218973"}]}